BoneJ2 does not calculate volume fraction of interpolated ROIs in DICOM files

[SSkull995]

Describe the bug
After converting my dcm files into a binary file, drawing different ROIs around the slices and interpolating them, when trying to calculate the BV/TV I always get random values. If I do not draw any ROI, I get the same value as if drawing and interpolating them. No change is observed. It seems boneJ does not calculate the BV/TV of the ROIs, but rather of the whole file

To Reproduce
Import DICOM
Convert to binary
Draw ROI/s and interpolate them
Go to BoneJ - Fraction - Area/Volume Fraction
The calculated BV/TV is wrong

Expected behavior
Calculation of BV and TV of the selected ROIs

Environment

• Fiji on MacOS
• Fiji on Win 11

[alessandrofelder]

Related to #164 - see also https://forum.image.sc/t/calculate-bone-volume-fraction-of-interpolated-rois/79820/2

[rmd13]

I have tried the BonJ1 to measure the Tk.Th and Tk.Sp with ROI as like the way you did.
What I foud is that, BonJ1 just crop the minimum stack out and do analysis, the pixels outside the roi still remains.
Thus in this view, I guess the ROI function does not make essential difference compared to BoneJ2–they only process a whole cube, not a sub fraction of volume.
So a better solution is to make the pixels outside roi all zeros, then the Area/Volume analysis or Tk.Th analysis will be fully targeted to the foregorund bone(ones).
To measure Tk.Sp, can fill all the pixels outside ROI as ones, thus only the bone marrow space (zeros) inside the roi will be analyzed for Tk.Sp.

See also from:
https://forum.image.sc/t/calculate-bone-volume-fraction-of-interpolated-rois/79820/9