diff --git a/src/Http/Controllers/GlideController.php b/src/Http/Controllers/GlideController.php
index 655fd4fa2fb..0e9fbcf9da4 100644
--- a/src/Http/Controllers/GlideController.php
+++ b/src/Http/Controllers/GlideController.php
@@ -16,6 +16,7 @@
 use Statamic\Facades\Config;
 use Statamic\Facades\Glide;
 use Statamic\Facades\Site;
+use Statamic\Facades\URL;
 use Statamic\Imaging\ImageGenerator;
 use Statamic\Support\Str;
 
@@ -218,7 +219,12 @@ private function validateSignature()
             return;
         }
 
-        $path = Str::after($this->request->url(), Site::current()->absoluteUrl());
+        $url = $this->request->url();
+        $siteUrl = Site::current()->absoluteUrl();
+
+        $path = Str::startsWith(Str::ensureRight($url, '/'), Str::ensureRight($siteUrl, '/'))
+            ? Str::after($url, $siteUrl)
+            : $this->removeSitePrefix($this->request->getPathInfo());
 
         try {
             SignatureFactory::create(Config::getAppKey())->validateRequest($path, $this->request->query->all());
@@ -226,4 +232,18 @@ private function validateSignature()
             abort(400, $e->getMessage());
         }
     }
+
+    /**
+     * Remove the longest site path prefix (e.g. /fr) from the path.
+     */
+    private function removeSitePrefix(string $path): string
+    {
+        $prefix = Site::all()
+            ->map(fn ($site) => Str::removeRight(URL::makeRelative($site->url()), '/'))
+            ->filter(fn ($prefix) => $prefix !== '' && Str::startsWith($path, $prefix.'/'))
+            ->sortByDesc(fn ($prefix) => strlen($prefix))
+            ->first();
+
+        return $prefix ? Str::after($path, $prefix) : $path;
+    }
 }
diff --git a/tests/Imaging/GlideTest.php b/tests/Imaging/GlideTest.php
index 4848b8816dc..018ad1d69ba 100644
--- a/tests/Imaging/GlideTest.php
+++ b/tests/Imaging/GlideTest.php
@@ -397,6 +397,53 @@ public function hybrid_caching_only_registers_the_cache_path_route()
         $this->get($signedUrl)->assertNotFound();
     }
 
+    #[Test]
+    #[DataProvider('signedUrlSiteProvider')]
+    public function it_validates_the_signature_against_the_site_url($siteUrl)
+    {
+        $this->setSites([
+            'en' => ['url' => $siteUrl, 'locale' => 'en_US'],
+        ]);
+
+        $url = $this->app->make(UrlBuilder::class)->build($this->createAsset(), ['w' => 100]);
+
+        $this->get($url)->assertOk()->streamedContent();
+    }
+
+    public static function signedUrlSiteProvider()
+    {
+        return [
+            'same domain' => ['http://localhost/'],
+            'different domain' => ['https://www.example.com/'],
+            'domain starting with the app domain' => ['http://local/'],
+        ];
+    }
+
+    #[Test]
+    #[DefineEnvironment('pathPrefixedSitesOnAnotherDomain')]
+    public function it_validates_the_signature_for_a_path_prefixed_site_on_a_different_domain()
+    {
+        Site::setCurrent('fr');
+
+        $url = $this->app->make(UrlBuilder::class)->build($this->createAsset(), ['w' => 100]);
+
+        $this->assertStringStartsWith('/fr/img/', $url);
+
+        $this->get($url)->assertOk()->streamedContent();
+    }
+
+    #[Test]
+    public function it_rejects_an_invalid_signature_when_the_site_url_is_on_a_different_domain()
+    {
+        $this->setSites([
+            'en' => ['url' => 'https://www.example.com/', 'locale' => 'en_US'],
+        ]);
+
+        $url = $this->app->make(UrlBuilder::class)->build($this->createAsset(), ['w' => 100]);
+
+        $this->get(str_replace('w=100', 'w=200', $url))->assertStatus(400);
+    }
+
     #[Test]
     public function cache_true_without_cache_path_will_throw_exception()
     {
@@ -619,6 +666,14 @@ private function fakeFfmpeg()
         });
     }
 
+    protected function pathPrefixedSitesOnAnotherDomain($app)
+    {
+        $app->booting(fn () => $this->setSites([
+            'en' => ['url' => 'https://www.example.com/', 'locale' => 'en_US'],
+            'fr' => ['url' => 'https://www.example.com/fr/', 'locale' => 'fr_FR'],
+        ]));
+    }
+
     protected function hybridCaching($app)
     {
         $app['config']->set('statamic.assets.image_manipulation.cache', 'hybrid');